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The precise role of glutamate dysregulation in OCD remains unclear, and we lack blinded, well-controlled studies demonstrating therapeutic benefit from glutamate-modulating agents

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Quantitative PCR To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 g was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs)

Dietary reference intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline [internet]